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Protein Structure - - Bog - Oxford University Press - Plusbog.dk

Protein Structure - - Bog - Oxford University Press - Plusbog.dk

New and updated, this second edition of Protein Structure presents electrophoretic, chromatographic, and spectrophotometric techniques as well as including new chapters on the relatively sophisticated methods of mass spectrometry and ultracentrifugation. The emphasis is not on the technical aspects of the instruments, but more on the practical aspects of sample preparation and the possibilities of experimental strategy and design. The companion volume to this book, Protein Function: A Practical Approach, details comparable simple techniques for characterizing protein function. Topics in this second volume include: protein molecular weight determination by SDS-PAGE; protein analysis by mass spectrometry; immunological detection of proteins of known sequence; identification of common post-translational modifications; peptide mapping; counting integral numbers of residues by chemical modification; disulfide bonds between cysteine residues; analysis of protein conformation by gel electrophoresis; hydrodynamic properties of proteins; determining the molar absorbance coefficient of a protein; optical spectroscopy to characterize protein conformation and conformational changes; measuring the conformational stability of a protein; imunochemical analysis of protein conformation; stabilization of protein structure by solvents. Protein Structure: A Practical Approach will prove invaluable to all researchers, from PhD students to experienced group leaders.

DKK 829.00
1

Protein Expression - - Bog - Oxford University Press - Plusbog.dk

Protein Purification Applications - - Bog - Oxford University Press - Plusbog.dk

Protein Purification Applications - - Bog - Oxford University Press - Plusbog.dk

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

DKK 870.00
1

Protein Purification Techniques - - Bog - Oxford University Press - Plusbog.dk

Protein Purification Techniques - - Bog - Oxford University Press - Plusbog.dk

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

DKK 616.00
1

Protein Engineering: A Practical Approach - - Bog - Oxford University Press - Plusbog.dk

Protein Science - Arthur (professor Of Biochemistry And Molecular Biology Lesk - Bog - Oxford University Press - Plusbog.dk

Protein Science - Arthur (professor Of Biochemistry And Molecular Biology Lesk - Bog - Oxford University Press - Plusbog.dk

Written primarily for students embarking on an undergraduate bioscience degree, this primer introduces students to the essential topics in protein science clearly and concisely by describing the basic chemical structure of proteins, the factors that stabilize protein structures, protein function, and protein evolution. It begins by placing proteins in their general context in life. They are synthesized as amino-acid sequences encoded in genomes, and fold spontaneously to three-dimensional structures. This is the point where life makes the tremendous leap from the one-dimensional world of genome and amino-acid sequences, to the three-dimensional world of protein structures - indeed, the world which we inhabit. Protein Science prepares readers for later more advanced study of the subject, but will also leave readers who do not go on to such advanced study with a satisfying grasp of the essentials of the subject.Protein Science is supported by online resources and is available for students and institutions to purchase in a variety of formats. The e-book offers a mobile experience and convenient access along with functionality tools, navigation features and links that offer extra learning support: www.oxfordtextbooks.co.uk/ebooks The online resources include:For students:- Self-test questions- Animations of protein structures introduced in the textFor registered adopters of the book:· Figures from the book, available to download

DKK 292.00
1

Guidebook to Molecular Chaperones and Protein-Folding Catalysts - - Bog - Oxford University Press - Plusbog.dk

Protein Structure and Function - Gregory A. (director Of The Rosentiel Center Petsko - Bog - Oxford University Press - Plusbog.dk

Introduction to Protein Science - Arthur M. (the Pennsylvania State University) Lesk - Bog - Oxford University Press - Plusbog.dk

Introduction to Protein Science - Arthur M. (the Pennsylvania State University) Lesk - Bog - Oxford University Press - Plusbog.dk

Over the last century, the centrality of proteins to life on earth has become increasingly clear - from forming enzymes acting as catalysts for specific reactions, to crucial molecules such as antibodies in the immune system, and as signalling molecules between cells. In fact, the more they are studied, the more proteins continue to reveal new and exciting functions. Indeed, by studying amino acid sequences and protein structures it is even possible to unravel some of the mysteries of evolution itself.Introduction to Protein Science, 3rd edition provides a rich and broad introduction to this fascinating field by covering not only the structure and function of proteins, but also the methods and experimental techniques used to study them. The practical applications of our knowledge to diverse fields such as biotechnology and medicine are emphasized throughout, to help students appreciate the relevance of the subject to the real world. Active learning is encouraged by the provision of frequent exercises and problems, with further ''weblems'' - web-based problems - on the Online Resource Centre.Online Resource CentreThe Online Resource Centre to accompany Introduction to Protein Science features:For students:·Answers to end-of-chapter exercises, and hints to end-of-chapter problems, so students can check their understanding.·Rotating structures from the book to help students visualize proteins in 3D.·Web-based problems for students to complete online.For registered adopters:·Figures from the book in electronic format, ready to download

DKK 616.00
1

Bioinformatics: Sequence, Structure and Databanks - - Bog - Oxford University Press - Plusbog.dk

Nature's Robots - Charles Tanford - Bog - Oxford University Press - Plusbog.dk

Nature's Robots - Charles Tanford - Bog - Oxford University Press - Plusbog.dk

Proteins are amazingly versatile molecules. They make the chemical reactions happen that form the basis for life, they transmit signals in the body, they identify and kill foreign invaders, they form the engines that make us move, and they record visual images. All of this is now common knowledge, but it was not so a hundred years ago. Nature''s Robots is an authoritative history of protein science, from the origins of protein research in the nineteenth century, when the chemical constitution of ''protein'' was first studied and heatedly debated and when there was as yet no glimmer of the functional potential of substances in the ''protein'' category, to the determination of the first structures of individual proteins at atomic resolution - when positions of individual atoms were first specified exactly and bonding between neighbouring atoms precisely defined. Tanford and Reynolds, who themselves made major contributions to the golden age of protein science, have written a remarkably vivid account of this history. It is a fascinating story, involving heroes from the past, working mostly alone or in small groups, usually with little support from formal research groups. It is also a story that embraces a number of historically important scientific controversies. Written in clear and accessible prose, Nature''s Robots will appeal to general readers with an interest in popular science, in addition to professional scientists and historians of science.

DKK 276.00
1

The Structure of Complex Networks - Ernesto Estrada - Bog - Oxford University Press - Plusbog.dk

The Structure of Complex Networks - Ernesto Estrada - Bog - Oxford University Press - Plusbog.dk

Matrix Metalloproteinases and TIMPs - Hideaki Nagase - Bog - Oxford University Press - Plusbog.dk

Hemoglobin - Jay F. (susan J. Rosowski Professor Of Biology Storz - Bog - Oxford University Press - Plusbog.dk

Hemoglobin - Jay F. Storz - Bog - Oxford University Press - Plusbog.dk

Phasing in Crystallography - Carmelo Giacovazzo - Bog - Oxford University Press - Plusbog.dk

Phasing in Crystallography - Carmelo Giacovazzo - Bog - Oxford University Press - Plusbog.dk

Modern crystallographic methods originate from the synergy of two main research streams, the small-molecule and the macro-molecular streams. The first stream was able to definitively solve the phase problem for molecules up to 200 atoms in the asymmetric unit. The achievements obtained by the macromolecular stream are also impressive. A huge number of protein structures have been deposited in the Protein Data Bank. The solution of them is no longer reserved to an elite group of scientists, but may be attained in a large number of laboratories around the world, even by young scientists. New probabilistic approaches have been tailored to deal with larger structures, errors in the experimental data, and modest data resolution. Traditional phasing techniques like ab initio, molecular replacement, isomorphous replacement, and anomalous dispersion techniques have been revisited. The new approaches have been implemented in robust phasing programs, which have been organized in automatic pipelines usable even by non-experts. Protein structures, which 50 years ago could take months or even years to solve, can now be solved in a matter of hours, partly also due to technological advances in computer science.This book describes all modern crystallographic phasing methods, and introduces a new rational classification of them. A didactic approach is used, with the techniques described simply and logically in the main text, and further mathematical details confined to the Appendices for motivated readers. Numerous figures and applicative details illustrate the text.

DKK 1066.00
1

Structure and Dynamics of Biomolecules - Peter A. Timmins - Bog - Oxford University Press - Plusbog.dk

Proteolytic Enzymes - - Bog - Oxford University Press - Plusbog.dk

Crystals, X-rays and Proteins - Dennis Sherwood - Bog - Oxford University Press - Plusbog.dk

Phage Display - - Bog - Oxford University Press - Plusbog.dk

This Book Just Ate My Dog! - Richard Byrne - Bog - Oxford University Press - Plusbog.dk

Shakespeare's Sonnets - - Bog - Oxford University Press - Plusbog.dk