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A Guide to Protein Isolation - C. Dennison - Bog - Springer - Plusbog.dk

A Guide to Protein Isolation - C. Dennison - Bog - Springer - Plusbog.dk

It is a truism of science that the more fundamental the subject, the more universally applicable it is. Nevertheless, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For -depth study of the mechanics of motor cars would tell one example, an in nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs",with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.

DKK 434.00
1

Plant Molecular Biology Manual - - Bog - Springer - Plusbog.dk

Plant Molecular Biology Manual - - Bog - Springer - Plusbog.dk

Designed for use in the classroom, this edition contains both fundamental and recently described techniques in the area of plant molecular biology. it presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis.

DKK 663.00
1

Immunochemistry in Clinical Laboratory Medicine - - Bog - Springer - Plusbog.dk

Immunochemistry in Clinical Laboratory Medicine - - Bog - Springer - Plusbog.dk

The rapid growth of specific protein estimations in the clinical laboratory over the last 10 years has been due to advances both in methodology and in the understanding of the role of the various plasma proteins in health and disease. This expansion has been made possible by the development of both gel phase and fluid phase techniques for the estimation of proteins and the ready availability of antisera to individual plasma proteins. The specificity of the immunological reaction has allowed the more precise identification and estimation of individual plasma proteins than was possible with dye binding or other chemical techniques, but at the same time these methods have introduced other possible errors and pitfalls. Advances in understanding of the structure and function of various plasma proteins has pointed the way to new clinical applications of plasma protein estimation in the diagnosis and monitoring of disease. The Symposium, of which these are the proceedings, was planned to bring together a number of experts in the field to discuss the available methods and their clinical application.

DKK 434.00
1

Advances in Clinical Nutrition - - Bog - Springer - Plusbog.dk

Advances in Clinical Nutrition - - Bog - Springer - Plusbog.dk

The advent of any new and effective therapy is soon followed by large numbers of publications in which the indications and benefits are explored critically. It is not unexpected, therefore, that within five years of the first Bermuda Symposium on advances in parenteral nutrition that a second Symposium was considered appropriate to review progress and explore new areas of investigation, as well as enlarging the scope of the meeting to include enteral nutrition. The rate of progress can be judged by the number of subjects which were not discussed at the first Symposium. For example, home parenteral nutrition, computer assisted assessment and prescribing, Studies of body protein synthesis and breakdown and the role of branched-chain amino acids are all new subjects for this Symposium which were not covered at all in the first meeting. Much progress has also been made to our understanding of the biochemi­ cal complications of parenteral nutrition and the problems related to long term access to the circulation. Nutritional care has become safer and more effective. There is an increasing awareness of the difficulties in making a true nutritional assessment in selecting patients for total parenteral nutrition and more attention has also been focussed on different approaches to enteral support in the management of undernourished patients. There is also continuing debate on the cost effectiveness of this expensive method of treatment and critics look in vain for evidence of efficacy based on controlled trials in specific groups of patients.

DKK 434.00
1