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Protein Folding - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Protein Folding - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

DKK 799.00
1

Single-Cell Protein Analysis - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Single-Cell Protein Analysis - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.

DKK 986.00
1

The Unfolded Protein Response - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

The Unfolded Protein Response - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

DKK 986.00
1

Methods of Protein Separation - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Methods of Protein Separation - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

DKK 663.00
1

Posttranslational Protein Modifications in the Reproductive System - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Posttranslational Protein Modifications in the Reproductive System - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This book''s aim is to increase the awareness of a great variety of posttranslational modifications in the male and female reproductive system. Some of the most intriguing reproductive strategies, mechanisms, and pathways involving PTM are discussed, with an added angle of evolutionary conservation and diversity. The book also chapters on sperm-egg binding, as well as on histone modification in both the embryo and sperm. Chapters are also devoted to protein ubiquitination, the regulation of sperm function during fertilization in mammals and tubulin modifications in gametes and embryos. There are no other current books on posttranslational protein modifications as they relate to reproduction, making this contribution unique in the field. It is useful for both researchers and graduate students alike.

DKK 795.00
1

DNA-Protein Interactions - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

DNA-Protein Interactions - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies.

DKK 986.00
1

Atlas of Protein Spectra in the Ultraviolet and Visible Regions - Donald M. Kirschenbaum - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Atlas of Protein Spectra in the Ultraviolet and Visible Regions - Donald M. Kirschenbaum - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Once you have seen the spectrum of one protein you have seen the spectra of all pro­ teins. Or so it would seem. While the general characteristics of the absorption curve may appear to be similar for all proteins (i. e. , in acid and neutral solution there is a minimum at 250 nm, a maximum at 278-282 nm, and no absorption above 310 nm; in alkaline solution the maximum and minimum shift to longer wavelengths), there are subtle differences which can be seen when the spectra of many proteins are compared. It is these differences which reflect changes in amino acid content and in the milieu in which the protein has been dissolved. The spectra in this book provide samples of these subtle spectral differences and permit comparisons to be made. This book was prepared to have its index read and its contents referred to. For the reader who desires to know what a protein spectrum looks like in acid and alkaline media, after X-ray or UV irradiation, or after photo-oxidation or B-bromosuccinimide treatment, spectral representations of all these experimental situations and many others are available. The indicies were prepared to provide the maximum information with the minimum effort. In addition to an alphabetical listing, all spectra are referred to by species, tissues, and the organs from which they were taken. There are also "environmental" indicies related to the treatment the proteins received prior to having their spectra taken. Technical information concerning instrumentation is lacking.

DKK 663.00
1

Plant Circadian Networks - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Plant Circadian Networks - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tagging of clock proteins and cellular imaging, protein level measurements, regulated RNA splicing detection, post-translational modifications, protein-protein interactions, and protein DNA/chromatin interactions. Additional chapters focus on seasonal processes like hypocotyl growth and flowering time control in annuals and trees, and techniques on soybean. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Plant Circadian Networks: Methods and Protocols, Second Edition is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general. "Period estimation and rhythm detection in timeseries data using BioDare2, the free, online, community resource" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

DKK 986.00
1

Soybeans - Keshun Liu - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Soybeans - Keshun Liu - Bog - Springer-Verlag New York Inc. - Plusbog.dk

The soybean [Glycine max (L. ) Merrill], a native of China, is one of the oldest crops of the Far East. For centuries, the Chinese and other Oriental people, including Japanese, Korean, and Southeast Asians, have used the bean in various forms as one of the most important sources of dietary protein and oil. For this reason and because the amount of protein produced by soybeans per unit area of land is higher than that of any other crop, this little old bean has been called "yellow jewel," "great treasure," "nature''s miracle protein," and "meat of the field. " Now this bean is seen by some as a weapon against world hunger and a protein of the future. Most recently, the soybean has been touted as a possible weapon against chronic diseases. Since large-scale introduction to the Western world at the beginning of the twentieth century, the cultivation and use of soybeans have undergone a dramatic revolution: from traditional soyfoods in the Orient to a new generation of soyfoods in the West, from animal feed to value-added food protein ingredients, from industrial paints to affordable table oils and spreads, from an old field crop to a new crop with wide regions of adoptability, herbicide tolerance, pest resistance, and/or altered chemical composition, and from limited regional cultivation to expanded worldwide production.

DKK 434.00
1

Food Allergens - Marija Gavrovic Jankulovic - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Food Allergens - Marija Gavrovic Jankulovic - Bog - Springer-Verlag New York Inc. - Plusbog.dk

A food allergen has the ability to first elicit an IgE response, and then, on subsequent exposures, a clinical response to the same or similar protein. How harmless food protein becomes recognized by the mucosal immune system as an allergen remains an open question and more data are needed to explain how regulatory mechanisms of the mucosal immune system fail and result in allergic sensitization to dietary antigens. Some biochemical characteristics associated with food allergens, such as the presence of multiple, linear IgE-binding epitopes and the resistance of the protein to digestion and processing, seem to predominate among food allergens. Digestion susceptibility of food allergens that sensitize via the gastrointestinal tract and stability to food processing conditions are inherently related to protein structural features. Thereby, physiological changes in the digestion process, pathological conditions affecting digestion, as well as procedures and food processing conditions that affect protein structure may all have a profound effect on the sensitizing potential and allergenicity of food proteins. In addition, signals coming from the diet and micro biome can modulate regulatory mechanisms of the mucosal immune system and influence mucosal immunity and intestinal barrier function. The detection of allergenic ingredients in food products has received increased attention from the food industry and legislative and regulatory agencies over recent years. This has resulted in the improvement of applied safety measures that provide protection for food-allergic consumers and development of sensitive and highly specific analytical methods of food allergens detection. Food allergy is an important and common health issue and therefore there is a need to characterize the sensitizing potential of newly introduced proteins in genetically engineered foods. A combination of in vitro and in silico methods provide information that contributes to safety assessment. Suitable in vivo models may provide a more holistic assessment of allergenic potential of novel food proteins.

DKK 780.00
1

The Pathological Protein - Philip Yam - Bog - Springer-Verlag New York Inc. - Plusbog.dk

The Pathological Protein - Philip Yam - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Prions are an entirely new class of pathogens, and scientists are just beginning to understand them. Although they have plagued humans and animals for 3 centuries, only in the last 2 decades have researchers linked them to diseases like Mad Cow. This book tells the strange story of their discovery, and the medical controversies that swirl around them. The author, Philip Yam, is a well-respected and connected journalist who is now an editor at Scientific American.

DKK 332.00
1

The Pathological Protein - Philip Yam - Bog - Springer-Verlag New York Inc. - Plusbog.dk

The Pathological Protein - Philip Yam - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Prions are an entirely new class of pathogens, and scientists are just beginning to understand them. Although they have plagued humans and animals for 3 centuries, only in the last 2 decades have researchers linked them to diseases like Mad Cow. This book tells the strange story of their discovery, and the medical controversies that swirl around them. The author, Philip Yam, is a well-respected and connected journalist who is now an editor at Scientific American.

DKK 264.00
1

Histone Methyltransferases - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Histone Methyltransferases - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume provides methods used to investigate histone methyltransferase function. Chapters guide readers through a comprehensive set of approaches that detail phylogenetic diversity, histone demethylase activities in vitro, generating chromatin substrates, auto-methylation, quantification of metabolites, protein purification, crystallization, X-ray structure, cryogenic electron microscopy, assessing genome-wide patterns, CUT&Tag in mouse embryonic tissues, chemical biology approaches, peptide SPOT arrays, nascent chromatin capture, ectopic protein tethering, computational models, and development of methyltransferase inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Histone Methyltransferases: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

DKK 986.00
1

cAMP Signaling - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

cAMP Signaling - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learning more about this important and developing field. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

DKK 986.00
1

MHC Molecules: Expression, Assembly and Function - Robert G. Urban - Bog - Springer-Verlag New York Inc. - Plusbog.dk

MHC Molecules: Expression, Assembly and Function - Robert G. Urban - Bog - Springer-Verlag New York Inc. - Plusbog.dk

3 nant expression systems have been used to make MHC molecules con­ taining a single peptide of interest. To date, fifteen single peptide class I structures (incorporating three different HLA and two different H-2 allotypes/isotypes) and four additional class II structures (two single peptide complexes and two superantigen complexes) have been reported. These advances have enabled us to study the atomic detail of antigen presentation and the general mechanisms behind peptide binding, and begin to construct models of T cell recognition. Another area of research which has exploded over the past five years has been the identification of MHC-associated peptides. There are several methods one can use to determine the sequence identity of MHC restricted peptides. Historically, the most successful technique, albeit crude and encumbered with serious limitations, has been the use of overlapping synthetic peptides and T cell clones. Unfortunately, this method absolutely requires: (i) knowledge of the target antigen; (ii) availability of T cell clones; and (iii) a relatively short overall length for the target source protein, such that a set of overlapping pep tides can be affordably synthesized. Briefly, the entire sequence of the tar­ get protein is chemically synthesized using overlapping peptides which are then screened for biological activity using standard T cell presen­ tation assays. Despite its limitations, this method was used to identify the first immunodominant epitopes reported in the literature and con­ tinues to be used successfully today.

DKK 663.00
1

DNA Damage Responses - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

DNA Damage Responses - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume provides detailed methods and key approaches used to m echanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

DKK 986.00
1

Chromatin - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Chromatin - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume provides cutting-edge techniques to further the study chromatin biology. Chapters include both novel and well-established methods for the analysis of DNA-associated proteins, DNA methylation, three-dimensional chromatin interactions, deep sequencing-based tools, and data analysis pipelines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, provides details of the necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and describes step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromatin: Methods and Protocols aims to further the understanding of how modified DNA and associated proteins affect the transcriptional output of the genome. Chapter Genome-wide mapping and microscopy visualization of protein-DNA interactions by pA-DamID [Chapter 12] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

DKK 986.00
1

Endocrinology of Pregnancy - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Endocrinology of Pregnancy - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

A comprehensive, cutting-edge review of the complex interactions between maternal and fetal-placental tissues that control the establishment and maintenance of pregnancy, the proper development of the fetus, the birth process, and the behavioral aspects of bonding between mother and newborn. Expert researchers review the endocrine and physiological events that culminate in the delivery of offspring, and provide a solid base of comparative information on the menstrual cycle of primates, including humans. They also discuss the sources and functions of both steroid and protein hormones from the placenta and the details of their effects on uterine function, placental development, fetal growth and well-being, and maternal responses to pregnancy. This book will become the standard reference source not only for reproductive scientists, but also for those clinicians who want better to understand the complex factors that affect pregnancy-and their pregnant patients.

DKK 901.00
1

Fibrous Proteins - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Fibrous Proteins - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This detailed volume explores fibrous proteins widely present in different biological tissues or biological structural materials. The book begins by introducing the structure of representative fibrous proteins, including animal silks, collagen, elastin, resilin, and keratin, and it then continues by providing detailed experimental protocols for the synthesis, assembly, and characterization of natural, regenerated, and recombinant fibrous proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fibrous Proteins: Design, Synthesis, and Assembly is an ideal guide for researchers aiming to master fibrous protein preparations with the aid of this broad and interdisciplinary perspective on understanding the structure-property-function relationships of natural and reconstituted fibrous proteins.

DKK 986.00
1

Mitosis - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Mitosis - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time.

DKK 986.00
1

Omics Approaches to Understanding Muscle Biology - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Omics Approaches to Understanding Muscle Biology - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This book is a collection of principles and current practices in omics research, applied to skeletal muscle physiology and disorders. The various sections are categorized according to the level of biological organization, namely, genomics (DNA), transcriptomics (RNA), proteomics (protein), and metabolomics (metabolite). With skeletal muscle as the unifying theme, and featuring contributions from leading experts in this traditional field of research, it highlights the importance of skeletal muscle tissue in human development, health and successful ageing. It also discusses other fascinating topics like developmental biology, muscular dystrophies, exercise, insulin resistance and atrophy due to disuse, ageing or other muscle diseases, conveying the vast opportunities for generating new hypotheses as well as testing existing hypotheses by combining high-throughput techniques with proper experiment designs, bioinformatics and statistical analyses. Presenting the latest research techniques, this book is a valuable resource for the physiology community, particularly researchers and grad students who want to explore the new opportunities for omics technologies in basic physiology research.

DKK 816.00
1

Post-Transcriptional Gene Regulation - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

Post-Transcriptional Gene Regulation - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA modifications, the book guides molecular biologists toward harnessing the power of this new generation of techniques, while also introducing the data analysis skills that these high-throughput techniques require. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Post-Transcriptional Gene Regulation, Third Edition serves as a versatile resource for researchers studying post-transcriptional regulation by both introducing the most recent techniques and providing a comprehensive guide to their implementation. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

DKK 816.00
1