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Protein Structure - - Bog - Oxford University Press - Plusbog.dk

New and updated, this second edition of Protein Structure presents electrophoretic, chromatographic, and spectrophotometric techniques as well as including new chapters on the relatively sophisticated methods of mass spectrometry and ultracentrifugation. The emphasis is not on the technical aspects of the instruments, but more on the practical aspects of sample preparation and the possibilities of experimental strategy and design. The companion volume to this book, Protein Function: A Practical Approach, details comparable simple techniques for characterizing protein function. Topics in this second volume include: protein molecular weight determination by SDS-PAGE; protein analysis by mass spectrometry; immunological detection of proteins of known sequence; identification of common post-translational modifications; peptide mapping; counting integral numbers of residues by chemical modification; disulfide bonds between cysteine residues; analysis of protein conformation by gel electrophoresis; hydrodynamic properties of proteins; determining the molar absorbance coefficient of a protein; optical spectroscopy to characterize protein conformation and conformational changes; measuring the conformational stability of a protein; imunochemical analysis of protein conformation; stabilization of protein structure by solvents. Protein Structure: A Practical Approach will prove invaluable to all researchers, from PhD students to experienced group leaders.

DKK 829.00

1

Protein Engineering - - Bog - Nova Science Publishers Inc - Plusbog.dk

Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.

DKK 890.00

1

Principles of Protein–Protein Association - Professor Harold P Erickson - Bog - Institute of Physics Publishing - Plusbog.dk

Anyoneteaching physical biochemistry or structural biology will find this to be aconcise and thorough guide for their lectures on protein-protein association.Students and researchers will appreciate the clarity of presentation offundamental concepts, and the guided reading of informative classic papers.

DKK 1014.00

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Protein Folding - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

DKK 799.00

1

Protein and Energy - Kenneth Carpenter - Bog - Cambridge University Press - Plusbog.dk

The author reviews the long-standing debate over the relative merits of a high-protein versus a low-protein diet. At a time when the concern has resurfaced that over-consumption of protein in affluent societies may damage health, this book provides a fascinating historical perspective.

DKK 778.00

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New Research on Protein Synthesis - - Bog - Nova Science Publishers Inc - Plusbog.dk

In this book, the authors present current research from across the globe in the study of protein synthesis. Topics discussed in this compilation include protein synthesis elongation factors EF-Tu and eEF1A and their application in the improvement of heat tolerance in plants; myostatin function in muscle protein homeostasis and its link with the regulation of translation; and energy regeneration systems in cell free protein in vitro.

DKK 1034.00

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Soy Protein - Hector Gonzalez - Bog - Nova Science Publishers Inc - Plusbog.dk

In this book, the authors study the production methods, functional properties and food sources of soy protein. Topics discussed include the rheology, texture and functionality of soy protein isolate-based potato puree; research advances on the relationships among the processing techniques for soy protein; and soybean proteins applied to microencapsulation as wall materials.

DKK 1034.00

1

Protein Trafficking in Neurons - - Bog - Elsevier Science Publishing Co Inc - Plusbog.dk

Examines mechanisms of protein trafficking and the role of trafficking in neuronal functioning from development to plasticity to disease. This book is divided into seven sections that review mechanisms of protein transport, the role of protein trafficking in synapse formation, exo- and endocytosis, transport of receptors, and more.

DKK 917.00

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Macromolecular Protein Complexes IV - - Bog - Springer International Publishing AG - Plusbog.dk

This book covers the latest findings of a wide variety of viral, prokaryotic and eukaryotic macromolecular protein complexes and builds upon the solid macromolecular foundations established by previous volumes of the Subcellular Biochemistry series. Thus, an almost encyclopaedic coverage of the broad field of protein complex structure and function has been established. The 17 interesting chapters included in this book have been organised into four sections: Soluble Protein Complexes, Membrane Protein Complexes, Fibrous Protein Complexes and Viral Protein Complexes. Significant topics present here are: Fatty Acid Synthase, the Fork Protection Complex, Ribonucleotide Reductase, the Kinetochore, G proteins, the FtsEX Complex, the Kainate Receptor, the Photosystem I-antenna, the Mycobacterial Arabinofuranosyltransferases, the the Bacterial Flagellum, the Actomyosin Complex, Motile Cilia, SLS Collagen Polymorphic Structures, and the Reovirus Capsid and Polymerase. Up-dates/expansion of chapter topics present in earlier volumes are now included in chapters here, e.g., those on Ferritin-like proteins and the Multi-tRNA Synthetase. The book is richly illustrated throughout, the result of an impressive integration of structural data from X-ray crystallography and cryo-electron microscopy. The functional aspects of protein-protein interactions are also given a high priority.

DKK 816.00

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Protein Acetylation - - Bog - Humana Press Inc. - Plusbog.dk

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.

DKK 855.00

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Principles of Protein Engineering - Christin Peters - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Comprehensive Reference Work Detailing Basic and Advanced Information Surrounding Protein Engineering Protein engineering is a method of changing a protein sequence to achieve a desired result, such as a change in the substrate specificity or increased stability to the temperature, organic solvents, and/or extremes of pH or selectivity in catalytic reactions. Principles of Protein Engineering provides researchers the necessary fundamental knowledge about the protein engineering field and the rationale behind the techniques. Written by two experts with significant first-hand experience in the field, the work provides insight on protein synthesis, expression, structure elucidation, bioinformatics, directed evolution, stability, screening, and rational design. To aid in reader comprehension, the text is enhanced by short comments, feedback and personal anecdotes from pioneers and leaders in the field. Sample ideas covered in the work include: - - The fundamental knowledge of protein synthesis and expression - - Basic knowledge about alignment, homology modelling, dynamics of structure, and modeling enzyme kinetics - - The main protein engineering strategies, with a focus on directed evolution and rational design - - Differences between screening and selection, plus the main techniques for high-throughput screening Biochemists, biotechnologists, molecular biologists, and students in related programs of study can use Principles of Protein Engineering to attain both holistic and specific insight into the field of protein engineering and its numerous applications.

DKK 1040.00

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Protein Water Interactions - - Bog - Nova Science Publishers Inc - Plusbog.dk

This book is aimed at understanding which molecular parameters control the thermodynamics, structure, and functions of the protein-water systems. Proteins are one of the most important classes of biological molecules. Water binding (hydration or biological water) plays a crucial role in determining the structure, stability, and functions of proteins. Knowledge of processes occurring upon hydration or dehydration of protein macromolecules is very important in biotechnological and pharmaceutical applications of proteins such as their use as biocatalysts, biosensors, and selective adsorbents. There are essential differences between hydration and bulk water surrounding a protein. This means that a characterisation of the hydration of protein macromolecules requires elucidating the effects of both the protein on water and vice versa. Therefore, a quantitative estimation of the protein and water contributions to the thermodynamic functions of binary protein-water systems is of considerable fundamental importance and practical interest. This book describes the basic principles of a novel methodology to investigate the protein-water interactions. This methodology is based on the analysis of the excess thermodynamic functions of mixing. The thermodynamic properties (volume V, enthalpy H, entropy S, heat capacity Cp, and Gibbs free energy G) of a real binary water-protein system can be expressed in terms of the excess functions. They are the difference between the thermodynamic function of mixing in a real system and the value corresponding to an ideal system at the same temperature, pressure and composition. For an ideal system, all excess functions are zero. Deviations of the excess functions from zero indicate the extent to which the studied binary system is non-ideal due to strong specific interactions between components (ie: hydrogen bonding and charge-charge interactions).

DKK 890.00

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Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics - - Bog - John Wiley & Sons Inc - Plusbog.dk

An in-depth look at the latest research, methods, and applications in the field of protein bioinformatics This book presents the latest developments in protein bioinformatics, introducing for the first time cutting-edge research results alongside novel algorithmic and AI methods for the analysis of protein data.

DKK 983.00

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Protein Folding and Metal Ions - - Bog - Taylor & Francis Ltd - Plusbog.dk

The role of metal ions in protein folding and structure is a critical topic to a range of scientists in numerous fields, particularly those working in structural biology and bioinorganic chemistry, those studying protein folding and disease, and those involved in the molecular and cellular aspects of metals in biological systems. Protein Folding and Metal Ions: Mechanisms, Biology and Disease presents the contributions of a cadre of international experts who offer a comprehensive exploration of this timely subject at the forefront of current research. Divided into four sections, this volume: - - Provides case study examples of protein folding and stability studies in particular systems or proteins that comprise different metal ions of co-factors - Reviews the proteins that shuttle metal ions in the cell to a particular target metalloprotein - Illustrates how metal binding can be connected to pathological protein conformations in unrelated diseases, from cancer to protein deposition disorders such as Parkinson’s disease - Addresses protein redesign of metal-containing proteins by computational methods, folding simulation studies, and work on model peptides — dissecting the relative energetic contribution of metals sites to protein folding and stability - Together, the 13 chapters in this text cogently describe the state of the science today, illuminate current challenges, propose future possibilities, and encourage further study in this area that offers much promise especially with regard to novel approaches to the treatment of some of the most challenging and tragic diseases.

DKK 806.00

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Mass Spectrometry of Protein Interactions - - Bog - John Wiley & Sons Inc - Plusbog.dk

Mass spectrometry (MS) is the major analytical technology used for protein identification, sequencing, and quantitation, and is central to discoveries in proteomics, and is likely to be a major tool for the study of protein interactions in the coming years.

DKK 968.00

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Protein Misfolding, Aggregation and Disease - Tara L. Pukala - Bog - John Wiley & Sons Inc - Plusbog.dk

Protein Misfolding, Aggregation and Disease

DKK 923.00

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Expressed Protein Ligation - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

DKK 855.00

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Protein Purification Applications - - Bog - Oxford University Press - Plusbog.dk

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

DKK 870.00

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Introduction to Protein Structure - Carl Ivar Branden - Bog - Taylor & Francis Inc - Plusbog.dk

Introduction to Protein Structure provides an account of the principles of protein structure, with examples of key proteins in their biological context generously illustrated in full-color to illuminate the structural principles described in the text. The first few chapters introduce the general principles of protein structure both for novices and for non-specialists needing a primer. Subsequent chapters use specific examples of proteins to show how they fulfill a wide variety of biological functions. The book ends with chapters on the experimental approach to determining and predicting protein structure, as well as engineering new proteins to modify their functions.

DKK 791.00

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Protein and Peptide Analysis by LC-MS - - Bog - Royal Society of Chemistry - Plusbog.dk

This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in ''Proteomics'' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.

DKK 1133.00

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Single-Cell Protein Analysis - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.

DKK 986.00

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Protein Synthesis and Translational Control - John Wb Hershey - Bog - Cold Spring Harbor Laboratory Press,U.S. - Plusbog.dk

Protein Synthesis and Translational Control

DKK 844.00

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Protein Engineering: A Practical Approach - - Bog - Oxford University Press - Plusbog.dk

Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specificities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

DKK 1105.00

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Protein NMR - - Bog - Humana Press Inc. - Plusbog.dk

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise. Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

DKK 901.00

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Protein Structure Prediction - - Bog - Springer-Verlag New York Inc. - Plusbog.dk

This thorough new edition explores web servers and software for protein structure prediction and modeling that are freely available to the academic community.

DKK 967.00

1

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