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Protein Purification

Protein Purification

This second edition of Protein Purification provides a guide to the major chromatographic techniques including non-affinity absorption techniques affinity procedures non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic starting with the properties of water progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates and postgraduates who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification. Key Features * Guide to the major techniques used in protein purification * Includes flowcharts to help the reader slect the best purification strategy * Contains step-by-step protocols that guide the reader through each technique and its use * Includes exercises and solutions

GBP 66.99
1

The Protein Folding Problem

The Protein Folding Problem

Proteins in living systems carry out a great variety of specific functions each of which depends on the precise three-dimensional structure of a particular protein. Proteins are synthesized in the form of a flexible polypeptide chain that is capable of assuming a vast number of configurations; the transformation of this chain into a specific relatively rigid three-dimensional structure is called folding-a remarkable process of self-organization. It is known that the amino acid sequences of some proteins have sufficient information to determine their three-dimensional structures. There are other proteins whose folding requires additional information beyond that found in the sequence of the mature protein. This book introduces the central problem of folding mechanisms as well as a number of other closely related issues. This book is neither a textbook nor a treatise. Rather it is an attempt by several investigators to convey the excitement and challenges of those aspects of the folding problem in which they are actively engaged. The contributors give brief introductions to protein folding from the perspectives of molecular architecture stability and dynamics phage genetics DNA exons general physiology and natural selection. They point out emerging new directions including the suggestion of a class of diseases that result from protein folding defects. | The Protein Folding Problem

GBP 130.00
1

Protein Actions Principles and Modeling

Protein Actions Principles and Modeling

Protein Actions: Principles and Modeling is aimed at graduates advanced undergraduates and any professional who seeks an introduction to the biological chemical and physical properties of proteins. Broadly accessible to biophysicists and biochemists it will be particularly useful to student and professional structural biologists and molecular biophysicists bioinformaticians and computational biologists biological chemists (particularly drug designers) and molecular bioengineers. The book begins by introducing the basic principles of protein structure and function. Some readers will be familiar with aspects of this but the authors build up a more quantitative approach than their competitors. Emphasizing concepts and theory rather than experimental techniques the book shows how proteins can be analyzed using the disciplines of elementary statistical mechanics energetics and kinetics. These chapters illuminate how proteins attain biologically active states and the properties of those states. The book ends with a synopsis the roles of computational biology and bioinformatics in protein science. | Protein Actions Principles and Modeling

GBP 99.99
1

The Fluorescent Protein Revolution

The Fluorescent Protein Revolution

Advances in fluorescent proteins live-cell imaging and superresolution instrumentation have ushered in a new era of investigations in cell biology medicine and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins The Fluorescent Protein Revolution explores the history properties and applications of these important probes. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers several cutting-edge imaging applications. These include superresolution microscopy of cellular fine structures FRET microscopy to visualize protein interactions and cell-signaling activities inside living cells photobleaching and photoactivation techniques to visualize protein behaviors techniques that exploit plant and algal photoreceptors to enable light-regulated control of enzymatic activities and the noninvasive imaging of tumor–host interactions in living animals. In color throughout this book presents the fundamental principles and latest advances in the field including the associated development of imaging techniques that exploit fluorescent proteins. It is accessible to a broad audience from optical imaging experts to novices needing an introduction to the field.

GBP 59.99
1

Peptide and Protein Drug Analysis

Peptide and Protein Drug Analysis

Furthering efforts to simulate the potency and specificity exhibited by peptides and proteins in healthy cells this remarkable reference supplies pharmaceutical scientists with a wealth of techniques for tapping the enormous therapeutic potential of these molecules-providing a solid basis of knowledge for new drug design. Provides a broad comprehensive overview of peptides and proteins as mediators of cell movement proliferation differentiation and communication. Written by more than 50 leading international authorities Peptides and Protein Drug Analysisdiscusses strategies for dealing with the complexity of peptides and proteins in conformational flexibility and amino acid sequence variability analyzes drug formulations facilitated by solid-phase peptide synthesis and recombinant DNA technology examines chemical purity analysis by high-pressure chromatographic capillary electrophoretic gel electrophoretic and isoelectric focusing methods highlights drug design elements derived from protein folding bioinformatics and computational chemistry demonstrates uses of unnatural mutagenesis and combinatorial chemistry explores mass spectrometry protein sequence and carbohydrate analysis illustrates bioassays and other new functional analysis methods surveys spectroscopic techniques such as ultraviolet fluorescence Fourier transform infrared and nuclear magnetic resonance (NMR) addresses ways of distinguishing between levels of therapeutic and endogenous agents in cells reviews structural analysis tools such as ultracentrifugation and light X-ray and neutron scattering and more! Featuring over 3400 bibliographic citations and more than 500 tables equations and illustrations Peptide and Protein Drug Analysis is a must-read resource for pharmacists; pharmacologists; analytical organic and pharmaceutical chemis

GBP 59.99
1

Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction Purification and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction purification and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations diagrams and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques the book also offers useful advice on which technique to use and when to apply a particular method. Presenting the advantages and disadvantages of the various protein techniques Principles and Reactions of Protein Extraction Purification and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.

GBP 205.00
1

Novel Plant Protein Processing Developing the Foods of the Future

Novel Plant Protein Processing Developing the Foods of the Future

Proteins serve as an important nutritional as well as structural component of foods. Not only do they provide an array of amino acids necessary for maintaining human health but also act as thickening stabilizing emulsifying foaming gelling and binding agents. The ability of a protein to possess and demonstrate such unique functional properties depends largely on its inherent structure configuration and how they interact with other food constituents like polysaccharides lipids and polyphenolic compounds. Proteins from animal sources have superior functionality higher digestibility and lower anti-nutrient components than plant proteins. However consumer preferences are evolving worldwide for ethically and sustainably sourced clean cruelty-free vegan or vegetarian plant-based food products. Unlike proteins from animal sources plant proteins are more versatile religiously and culturally acceptable among vegetarian and vegan consumers and associated with lower food processing waste water and soil requirement. Thus the processing and utilization of plant proteins have gained worldwide attention and as such numerous scientific studies are focusing on enhancing the utilization of plant proteins in food and pharmaceutical products through various processing and modification techniques to improve their techno-functional properties bioactivity bioavailability and digestibility. Novel Plant Protein Processing: Developing the Foods of the Future presents a roadmap for plant protein science and technology which will focus on plant protein ingredient development plant protein modification and the creation of plant protein-based novel foods. Key Features: Includes complete information about novel plant protein processing for use as future foods Presents a roadmap to upscale the meat analog technological processes Discusses marketing limitations of plant based proteins and future opportunities This book highlights the important scientific technological advancements that are being deployed in the future foods using plant proteins concerns opportunities and challenges and as an alternative to maintaining a healthy and sustainable modern food supply. It covers the most recent research related to the plant protein-based future foods which include their extraction isolation modification characterization development and final applications. It also covers the formulation and challenges: emphasis on the modification for a specific use legal aspects business perspective and future challenges. This book is useful for researchers readers scientists and industrial people to find information easily. | Novel Plant Protein Processing Developing the Foods of the Future

GBP 170.00
1

Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

The Latest Developments on the Role of Dynamics in Protein Functions Computational Approaches to Protein Dynamics: From Quantum to Coarse-Grained Methods presents modern biomolecular computational techniques that address protein flexibility/dynamics at all levels of theory. An international contingent of leading researchers in chemistry physics and biology show how these advanced methods provide insights into dynamic aspects of biochemical processes. A particular focus is on intrinsically disordered proteins (IDPs) which lack a well-defined three-dimensional structure and function as dynamic ensembles. The book covers a wide spectrum of dynamics from electronic structure-based to coarse-grained techniques via multiscaling at different levels. After an introduction to dynamics and historical overview of basic methodologies the book addresses the following issues: Is there a quantitative relationship between enzymatic catalysis and protein dynamics? Which are the functionally relevant motions of proteins? How can structural properties and partner recognition mechanisms of IDPs be simulated? How can we speed up molecular dynamics? How can we describe conformational ensembles by the synergistic effort of computations and experiments? While dynamics is now considered essential for interpreting protein action it is not yet an integral component in establishing structure–function relationships of proteins. Helping to reshape this classical view in biochemistry this groundbreaking book explores advances in computational methodology and contributes to the new ensemble way of studying proteins. | Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

GBP 69.99
1

Biochemistry in the Lab A Manual for Undergraduates

Biochemistry in the Lab A Manual for Undergraduates

Most lab manuals assume a high level of knowledge among biochemistry students as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching together with a complete experimental experience Includes chemical biology as its foundation teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute at comparatively low cost Supersedes existing older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner as well as several modules to study protein chemistry including gel techniques enzymology crystal growth unfolding studies and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute at comparatively low cost. | Biochemistry in the Lab A Manual for Undergraduates

GBP 48.99
1

Protein Calories And Development Nutritional Variables In The Economics Of Developing Countries

Protein Calories And Development Nutritional Variables In The Economics Of Developing Countries

Production of world food supplies is related to more complicated socioeconomic variables than have previously been analyzed. Besides traditional inputs of land labor and fertilizer the technological capabilities and a variety of nutritional and other human capital components are significant independent variables in explaining agricultural production in the developing world. The integration of economic analyses with the concepts of nutritional science offers an expanded and effective means for analyzing the complex problems of agricultural production in nutritionally deficient countries. Bernard Schmitt traces the circular relationship between nutrition and human capital labor productivity food production and per capita consumption of calories and protein. He defines the basic nutritional terms that are most useful to economists in analyzing agricultural and foodrelated questions and provides examples that stress the importance of concentrating on nutritional quality as well as gross quantity. Transformations are used to convert quantities into basic nutritional components allowing more meaningful quantitative analyses in an econometric framework. Dr. Schmitt presents a flexible methodology for forecasting commodity production using it to make projections for the developing countries for each major commodity group and to test various policy alternatives such as extensive trade expanded food assistance programs substantial resource or input expansion further expansion of Green Revolution technology and development of alternatives to agriculture. Although he is certain that gains can be accomplished through population control and agricultural advances supplemented by alternative nutritional sources he concludes that conditions in nutritionally deficient countries are unlikely to improve on average through the mid-1980s. | Protein Calories And Development Nutritional Variables In The Economics Of Developing Countries

GBP 125.00
1

Environmental Chemistry in the Lab

Environmental Chemistry in the Lab

Environmental Chemistry in the Lab presents a comprehensive approach to modern environmental chemistry laboratory instruction together with a complete experimental experience. The laboratory experiments have an introduction for the students to read a pre-lab for them to complete before coming to the lab a data sheet to complete during the lab and a post-lab which would give them an opportunity to reinforce their understanding of the experiment completed. Instructor resources include a list of all equipment and supplies needed for 24 students a lab preparation guide an answer key to all pre-lab and post-lab questions sample data for remote learners and a suggested rubric for grading the labs. Additional features include: • Tested laboratory exercises with instructor resources for environmental science students • Environmental calculations industrial regulation and environmental stewardship • Classroom and remote exercises • An excellent user-friendly and thought-provoking presentation which will appeal to students with little or no science background • A qualitative approach to the chemistry behind many of our environmental issues today

GBP 56.99
1

Intracellular Calcium-Dependent Proteolysis

Intracellular Calcium-Dependent Proteolysis

Intracellular Calcium-Dependent Proteolysis explains what is now known about calpains which are intracellular non-lysosomal enzymes involved in intracellular protein catabolism. The book provides a comprehensive overview of topics ranging from the molecular biology of the calpains and their specific inhibitor protein (calpastatin) to physiologic and pathologic consequences of the presence of this proteolytic system in many model cells and tissues. Several theoretical functions of the calpains are discussed including their potential roles in muscle protein turnover platelet activation membrane fusion and synaptic plasticity. Intracellular Calcium-Dependent Proteolysis is a valuable source of information for researchers and students interested in the regulation of intracellular protein catabolism and the general effects of Ca2+ on cell function.

GBP 180.00
1

Phage Display In Biotechnology and Drug Discovery

Phage Display In Biotechnology and Drug Discovery

Phage Display in Biotechnology and Drug Discovery Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition the book discusses current theories principles and methods in the field and demonstrates applications for peptide phage display protein phage display and the development of novel antibodies. The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery. Key Features: Describes the basic principles of phage display and the methods and systems that have been developed for key applications Outlines applications for peptide phage display in the development of vaccines and high- throughput screens as well as the exploration of protein–protein interactions and enzyme substrate characteristics Details the use of phage display in the engineering of protein stability the identification and mapping of protein–protein interactions and the development of catalysts Provides broad coverage of the impact of phage display technology on the development of protein therapeutics Presents expert opinions on future challenges in the field

GBP 69.99
1

Introduction to Computational Proteomics

Introduction to Computational Proteomics

Introduction to Computational Proteomics introduces the field of computational biology through a focused approach that tackles the different steps and problems involved with protein analysis classification and meta-organization. The book starts with the analysis of individual entities and works its way through the analysis of more complex entities from protein families to interactions cellular pathways and gene networks. The first part of the book presents methods for identifying the building blocks of the protein space such as motifs and domains. It also describes algorithms for assessing similarity between proteins based on sequence and structure analysis as well as mathematical models such as hidden Markov models and support vector machines that are used to represent protein families and classify new instances. The second part covers methods that investigate higher order structure in the protein space through the application of unsupervised learning algorithms such as clustering and embedding. The book also explores the broader context of proteins. It discusses methods for analyzing gene expression data predicting protein-protein interactions elucidating cellular pathways and reconstructing gene networks. This book provides a coherent and thorough introduction to proteome analysis. It offers rigorous formal descriptions along with detailed algorithmic solutions and models. Each chapter includes problem sets from courses taught by the author at Cornell University and the Technion. Software downloads data sets and other material are available at biozon. org

GBP 59.99
1

Nanotechnology in Drug Delivery Fundamentals Design and Applications

Nanotechnology in Drug Delivery Fundamentals Design and Applications

This important new book provides the fundamental understanding of the peptide and protein drug delivery systems with a special focus on their nanotechnology applications. Addressing an increasing interest in peptide and protein drug delivery systems in both academic and industrial circles worldwide this book fills the need for a comprehensive review and assessment of conventional and nonconventional routes of administration. | Nanotechnology in Drug Delivery Fundamentals Design and Applications

GBP 82.99
1

Designer’s Guide to Lab Practice

Designer’s Guide to Lab Practice

This book explores the growing field of bio-design through interdisciplinary creative practice. The volume illustrates a range of experimental working techniques while offering a foundational understanding of lab practice principles. The book highlights the myriad of opportunities presented by microorganisms that have reshaped the planet and made it habitable. The book provides an account of the creation of living materials from the point of view of an architectural design practitioner. The transition from traditional design practice to laboratory investigation is captured highlighting strategies of creating partnerships across a range of fields. The book demonstrates laboratory methods and ways of investigating the development of living materials and celebrates the growing body of practitioners scientists activists and anthropologists who are reimagining new strategies for addressing contemporary environmental challenges. Designer's Guide to Lab Practice looks at ways in which integrating living components with needs of their own would not only help offset the environmental impact that we have on our planet but could also create a closer relationship with nature. It is a working manual as well as a guide to emerging practitioners seeking to transition into a field that is yet to be defined and that offers the promise of a new era of human habitat making as a direct response to the looming ecological crisis. | Designer’s Guide to Lab Practice

GBP 31.99
1

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced and processes seamlessly integrated. This microscale advantage translates into greater sensitivity more accurate results and better information. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology its limitations and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods microfluidic operations chemical separations sample preparation and injection methods detection technology and various chemical and biological analyses. Other topics of interest include the use of polymeric chips fluid flow valve and control single-cell analysis DNA and RNA amplification techniques DNA hybridization immunoassays and enzymatic assays. Originally conceived as a single chapter published in Ewing's Analytical Instrumentation this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.

GBP 44.99
1

Knowledge Discovery in Proteomics

Knowledge Discovery in Proteomics

Multi-modal representations the lack of complete and consistent domain theories rapid evolution of domain knowledge high dimensionality and large amounts of missing information - these are challenges inherent in modern proteomics. As our understanding of protein structure and function becomes ever more complicated we have reached a point where the actual management of data is a major stumbling block to the interpretation of results from proteomic platforms to knowledge discovery. Knowledge Discovery in Proteomics presents timely authoritative discussions on some of the key issues in high-throughput proteomics exploring examples that represent some of the major challenges of knowledge discovery in the field. The authors focus on five specific domains:Mass spectrometry-based protein analysisProtein-protein interaction network analysisSystematic high-throughput protein crystallizationSystematic integrated analysis of multiple data repositoriesSystems biologyIn each area the authors describe the challenges created by the type of data produced and present potential solutions to the problem of data mining within the domain. They take a systems approach covering individual data and integrating its computational aspects from data preprocessing storage and access to analysis visualization and interpretation. With clear exposition practical examples and rich illustrations this book presents an outstanding overview of this emerging field and builds the background needed for the fruitful exchange of ideas between computational and biological scientists.

GBP 59.99
1

Advanced Mammalian Cell Culture Techniques Principles and Practices

Advanced Mammalian Cell Culture Techniques Principles and Practices

This up-to-date book compiles both basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture nucleic acid and protein isolation cell-staining techniques and cell transfection and single-cell analysis. The topics include aseptic handling media preparation and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures. Key Features: Covers cellular staining using fluorescent dyes genetic manipulation of cells via transfection and an introduction to single-cell analyses Discusses basics in cell culture and downstream applications including gene and protein expression analysis Includes the principles underlying each of the techniques and provides a detailed methodology to practice Explores the whole range of techniques – from basic to downstream applications and advanced methods The book is essential for students and researchers in the field of life sciences biotechnology genetics and molecular biology. | Advanced Mammalian Cell Culture Techniques Principles and Practices

GBP 89.99
1