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Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction Purification and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction purification and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations diagrams and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques the book also offers useful advice on which technique to use and when to apply a particular method. Presenting the advantages and disadvantages of the various protein techniques Principles and Reactions of Protein Extraction Purification and Characterization enables students and researchers to master the mechanisms behind the protocols and choose the best method for their purposes.

GBP 205.00
1

Intracellular Calcium-Dependent Proteolysis

Intracellular Calcium-Dependent Proteolysis

Intracellular Calcium-Dependent Proteolysis explains what is now known about calpains which are intracellular non-lysosomal enzymes involved in intracellular protein catabolism. The book provides a comprehensive overview of topics ranging from the molecular biology of the calpains and their specific inhibitor protein (calpastatin) to physiologic and pathologic consequences of the presence of this proteolytic system in many model cells and tissues. Several theoretical functions of the calpains are discussed including their potential roles in muscle protein turnover platelet activation membrane fusion and synaptic plasticity. Intracellular Calcium-Dependent Proteolysis is a valuable source of information for researchers and students interested in the regulation of intracellular protein catabolism and the general effects of Ca2+ on cell function.

GBP 180.00
1

Uses of Elemental Diets in Clinical Situations

Uses of Elemental Diets in Clinical Situations

The studies presented in this book demonstrate that a new concept in the management of carious intestinal disorders should be considered namely that common nutrients may protect or heal the mucosa by virtue of the particular form and mode in which they are delivered to the intestinal lumen and their availability to the mucosal cells. The substitution of intact protein in the formula by hydrolysate appears to me an important factor. | Uses of Elemental Diets in Clinical Situations

GBP 205.00
1

Receptor Phosphorylation

Receptor Phosphorylation

The following chapters comprise invited contributions from eminent scientists who are internationally recognized authorities on the subject. The chapters have been written to provide the reader with adequate background necessary experimental details and discussion that is easy to comprehend. The book has been organized into four different sections. The introductory chapter is meant to briefly summarize the contributions of other authors and discuss phosphorylation of receptor systems not covered in the book in detail. The chapters in the second section on protein kinases and phosphatases are quite fundamental to the process of phosphorylation and will aid the reader in appreciating the observations and discussions reported in other chapters. The third and fourth sections present discussions on phosphorylation of various receptor systems which are involved in mediating actions of peptide amine and steroid hormones. It is hoped that this book will serve as a valuable resource and will be useful to all workers and students in the area of receptor-ligand interactions. | Receptor Phosphorylation

GBP 205.00
1

Organelle and Molecular Targeting

Organelle and Molecular Targeting

We have surpassed the omics era and are truly in the Age of Molecular Therapeutics. The fast-paced development of SARS-CoV-2 vaccines such as the mRNA vaccines encoding the viral spike protein demonstrated the need for and capability of molecular therapy and nanotechnology-based solutions for drug delivery. In record speed the SARS-CoV-2 viral RNA genome was sequenced and shared with the scientific community allowing the rapid design of molecular therapeutics. The mRNA vaccines exploit the host cell endoplasmic reticulum to produce viral spike proteins for antigen presentation and recognition by the innate and adaptive immune system. Lipid nanoparticles enable the delivery of the fragile degradation-sensitive nucleic acid payloads. Molecular-based therapeutics and nanotechnology solutions continue to drive the scientific and medical response to the COVID-19 pandemic as new mRNA DNA and protein-based vaccines are developed and approved and the emergency use approved vaccines are rapidly manufactured and distributed throughout the globe. The need for molecular therapies and drug delivery solutions is clear and as these therapies progress and become more specialized there will be important advancements in organelle targeting. For example using organelle targeting to direct lipid nanoparticles with mRNA payloads to the endoplasmic reticulum would increase the efficacy of mRNA vaccines reducing the required dose and therefore the biomanufacturing demand. Likewise improving the delivery of DNA therapeutics to the nucleus would improve efficacy. Organelles and molecules have always been drug targets but until recently we have not had the tools or capability to design and develop such highly specific therapeutics. Organelle targeting has far-reaching implications. For example mitochondria are central to both energy production and intrinsic apoptosis. Effectively targeting and manipulating mitochondria has therapeutic applications for diseases such as myopathies cancer neurodegeneration progerias diabetes and the natural aging process. The SARS-CoV-2 vaccines that exploit the endoplasmic reticulum (for mRNA vaccines) and the nucleic translational process (DNA vaccines) attest to the need for organelle and molecular therapeutics. This book covers the status demand and future of organelle- and molecularly targeted therapeutics that are critical to the advancement of modern medicine. Organelle and molecular targeting is the drug design and drug delivery approach of today and the future; understanding this approach is essential for students scientists and clinicians contributing to modern medicine.

GBP 200.00
1

Bioactive Peptides Production Bioavailability Health Potential and Regulatory Issues

Bioactive Peptides Production Bioavailability Health Potential and Regulatory Issues

Bioactive peptides have been receiving attention recently due to their applications as health-promoting agents. Derived from food proteins and other natural sources they exhibit various beneficial effects such as preventing diseases or modulating physiological systems once absorbed. As the market for nutraceuticals and functional foods continues to expand consumer interest has also grown and there are many common foods that have shown an abundance of bioactive peptides including dairy products cereal legumes meat and numerous other sources. In this newest addition to the series Nutraceuticals: Basic Research and Clinical Applications Bioactive Peptides: Production Bioavailability Health Potential and Regulatory Issues provides a comprehensive review of the current state of knowledge in the field of food protein hydrolysates and bioactive peptides their food sources bioavailability production applications functionalities health potentials and regulatory issues governing their use. Features Discusses different methodologies employed for scaling up bioactive peptides commercially Provides information on optimizing the production process Explains various bioactive properties exerted by different types of bioactive peptides Explores the application of metabolomics to the study of bioactive peptides With over 20 chapters written by established subject matter experts in their field this book provides timely information and discusses the latest developments of bioactive peptides. It will be useful for researchers academics and industry experts and can serve as an excellent resource for anyone interested in enhancing their knowledge in the field of bioactive peptides. | Bioactive Peptides Production Bioavailability Health Potential and Regulatory Issues

GBP 190.00
1